| Shortcut | Action |
| F5 | Previous spotscan |
| F6 | Next spotscan |
| F10 | Pick particles |
| F8 | Tiltpick particles |
| LEFT MOUSE | Add mask/particle/pair |
| RIGHT MOUSE | Remove mask/particle/pair |
| hold SHIFT | Hide particles |
| Alt | Hide pair numbers |
| CTRL + Mousewheel | Zoom spotscan |
| SHIFT + Mousewheel | Change particle size |
| Mousewheel | Change variance slider (“Variance threshold”) |
| MID MOUSE | Invert spotscan |
| CTRL + O | Open files |
| CTRL + L | Toggle linked panning |
| CTRL + D | Select dirt mode |
| CTRL + A | Select particle mode |
| CTRL + S | Select pair mode |
| CTRL + C | Clear overlapping particles |
| CTRL + M | Mass centering |
| CTRL + R | Toggle autopicking |
| CTRL + Y | Clear all particles |
| CTRL + X | Load plt file |