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tutorial [2017/06/13 10:14] dhaselb [Step 1: Import data] |
tutorial [2017/06/30 20:08] (current) kbertra2 [Step 2: Preprocessing] |
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| - //Coarsing:// Find the logic group **ImageOperations.** Click and drag the [[eyes:logics:BoxManipulation|]] logic into your workspace. In the **Properties** window, select "Coarse" and set the Coarse factor to 3. Connect the [[eyes:logics:BoxManipulation|]] logic to the [[eyes:logics:Import|]] logic. Note: if using multiple tabs in the GUI, simply click and drag the desired logic from the **History** window into the **Quick Access** window. Click the "Play" icon to run the new logic. {{ :selection_026.png |}} | - //Coarsing:// Find the logic group **ImageOperations.** Click and drag the [[eyes:logics:BoxManipulation|]] logic into your workspace. In the **Properties** window, select "Coarse" and set the Coarse factor to 3. Connect the [[eyes:logics:BoxManipulation|]] logic to the [[eyes:logics:Import|]] logic. Note: if using multiple tabs in the GUI, simply click and drag the desired logic from the **History** window into the **Quick Access** window. Click the "Play" icon to run the new logic. {{ :selection_026.png |}} | ||
| + | - //Filtering:// Find the logic group **ImageOperations.** Click and drag the [[eyes:logics:filtering|]] logic into your workspace. In the **Properties** window, apply the following settings: {{ ::selection_055.png |}} Connect the [[eyes:logics:filtering|]] logic with [[eyes:logics:BoxManipulation|]] of the previous step and click the "Play" icon to run the new logic. | ||
| - //Normalization:// Select [[eyes:logics:Normalization|]] from **ImageOperations** In **Properties** apply the settings shown below. Connect with [[eyes:logics:Filtering|]] from the previous step and click "Play." //The output of [[eyes:logics:Normalization|]] is your set of preprocessed particles.// {{ :selection_029.png |}} | - //Normalization:// Select [[eyes:logics:Normalization|]] from **ImageOperations** In **Properties** apply the settings shown below. Connect with [[eyes:logics:Filtering|]] from the previous step and click "Play." //The output of [[eyes:logics:Normalization|]] is your set of preprocessed particles.// {{ :selection_029.png |}} | ||
| ===== Step 3: 2D operations ===== | ===== Step 3: 2D operations ===== | ||
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| ==== Alignment with reference ==== | ==== Alignment with reference ==== | ||
| - | - Repeat steps 1-4 from **Generation of references** to generate another set of references. | + | - Repeat steps 1-4 from **Generation of references** to generate another set of references. Use the newest references each time but always use the original preprocessed particles. |
| - Use new references (i.e. the output of each iterative [[eyes:logics:MassAlignment|]]) as the "Align Infos" for the [[eyes:logics:Alignment|]] logic. | - Use new references (i.e. the output of each iterative [[eyes:logics:MassAlignment|]]) as the "Align Infos" for the [[eyes:logics:Alignment|]] logic. | ||
| - | - //Iteration:// Repeat steps 1- several times, but skip step 5 since a reference is created after each iteration. Use the newest references each time but always use the original preprocessed particles. | ||
| ===== Step 4: Dataset cleaning ===== | ===== Step 4: Dataset cleaning ===== | ||
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| ===== Step 6: Refinement ===== | ===== Step 6: Refinement ===== | ||
| - | - //3D Refinement//: Find the "3DOperations" logic group in the **Logics Panel** and select 3DRefinement. Apply the following settings: {{ ::selection_032.png |}} | + | //This Step is currently under construction.// |
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| - | FSC | + | |
| - | Sharpening | + | |