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eyes:logics:reconstruction [2017/06/12 14:03] vzinch [Concept] |
eyes:logics:reconstruction [2017/06/12 15:39] vzinch [Modes] |
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| Normalize 3D | Normalizes the obtained 3D structure (mean=0) | | | Normalize 3D | Normalizes the obtained 3D structure (mean=0) | | ||
| → 3D normalization sigma | Sigma value for normalization | | | → 3D normalization sigma | Sigma value for normalization | | ||
- | | Padding factor | FIXME | | + | | Padding factor | Factor by which the box is padded with zeroes in fourier space | |
| Already phase flipped | Indicate whether phase flipping was applied on the previous steps | | | Already phase flipped | Indicate whether phase flipping was applied on the previous steps | | ||
| Reproject 3D | Gives additional output of 2D reprojections of the obtained 3D structure | | | Reproject 3D | Gives additional output of 2D reprojections of the obtained 3D structure | | ||
| Symmetry | Symmetry of the complex studied (if known) | | | Symmetry | Symmetry of the complex studied (if known) | | ||
- | | With CTF | Indicate whether header files with CTF are available | | + | | With CTF | Indicate whether header values with CTF parameters are available | |
|< 100% 30% >| | |< 100% 30% >| | ||
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===== Concept ===== | ===== Concept ===== | ||
- | Real Space mode uses the [[http://www.owlnet.rice.edu/~elec539/Projects97/cult/node2.html|Filtered Back-Projection]] method, but applies 2D fitering instead of 1D, that reduces sensitivity to noise. | + | Real Space mode uses the [[http://www.owlnet.rice.edu/~elec539/Projects97/cult/node2.html|Filtered Back-Projection]] method, but applies 2D filtering instead of 1D, that reduces sensitivity to noise. |
- | Fourrier Space Mode uses Fourier inversion method based on [[https://en.wikipedia.org/wiki/Projection-slice_theorem|central slice theorem]]. | + | Fourier Space Mode uses Fourier inversion method based on [[https://en.wikipedia.org/wiki/Projection-slice_theorem|central slice theorem]]. |
- | More infomrtaion about the reconstruction in Real and Fourier space can be found in Chapter 7 of the following [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239172/pdf/cr100353t.pdf|review]]. | + | More information about the reconstruction in Real and Fourier space can be found in Chapter 7 of the following [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239172/pdf/cr100353t.pdf|review]]. |