====== FourierShellCorrelation ====== Fourier Shell Correlation (FSC) is the most commonly used method for resolution determination of a 3D structure reconstruction. ===== Usage ===== This logic applies the "gold standard" FSC calculation for resolution determination of a 3D structure reconstruction where the data had been split into two randomly chosen halves earlier. After independent reconstruction of both halves, the FSC fall-off at different resolution levels can be used to determine the reconstruction quality. Additionally, a mask can be supplied to limit the 3D volume to be calculated with. ===== Parameters and I/O ===== |< 100% 30% >| ^ Parameters ^ Description ^ | Calculate resolution | Picks the resolution mode to be used | | -> Plot threshold curves | Enabling results in all possible threshold curves being included in the output | | Mask | Enables use of an input mask | | -> invert mask | Invert the selected/not selected volume of the mask | | -> Mask Threshold | Threshold for counting mask voxel for mean calculation | | Asymmetric units (3 sigma) | Number of asymmetric units in the 3D | | Pixel size | Used pixel size in the 3Ds (Pixel/Angstrom) | |< 100% 30% >| ^ Input ^ Description ^ | First3D | First 3D Structure/Reconstructed 3D from first half of data | | Mask | Optional input, can be used to restrict 3D volume to only look at certain parts of the reconstruction | | Second3D | Second 3D Structure/Reconstructed 3D from second half of data | | FourierShellCorrelation | A Graph showing the calculated FSC (y-axis, ranging from 0 to 1) against resolution (x-axis, Angstrom) | |< 100% 30% >| ^ Output ^ Description ^ | FourierShellCorrelation | A Graph showing the calculated FSC (y-axis, ranging from 0 to 1) against resolution (x-axis, Angstrom) | |< 100% 30% >| ^ Written Header Values ^ Description ^ | resolutionAt0.143 | Estimated resolution for FSC cut off at 0.143 | | resolutionAt0.3 | Estimated resolution for FSC cut off at 0.3 | | resolutionAt0.5 | Estimated resolution for FSC cut off at 0.5 | | resolutionAt0.9 | Estimated resolution for FSC cut off at 0.9 | | resolutionAt3Sigma | Estimated resolution for FSC cut off at 3 Sigma | | resolution | Estimated resolution at the user chosen cut off value | | maxResolution | Maximum resolution in the local map | | meanResolution | Mean resolution in the local map | | minResolution | Minimum resolution in the local map | | resolutionVariance | Variance of the resolution in the local map | | abscissaIsLogarithmical , abscissaMin, abscissaMax | For internal 1D viewer use only, to be hidden | ===== Concept ===== A Paper about FSC theory and applications can be found [[http://www.sciencedirect.com/science/article/pii/S1047847705001292|here]]